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BriClone Hybridoma Cloning Additive

BRI Sample - BriClone Hybridoma Cloning Additive, Sample

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BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma cell cloningIt has been specifically developed by the NICB's dedicated team to overcome common problems in the post-fusion period, such as batch-to-batch variation of feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone is intended for use when cloning hybridoma cell lines under conditions of limiting dilution.  

BriClone is a conditioned medium collected from a proprietary human cell line that produces IL-6. It is supplied in a sterile frozen format. Each batch of BriClone is tested for its ability to support the outgrowth of hybridomas from freshly fused cells and for its ability to support the growth of hybridomas under conditions of limiting dilution.

To use BriClone, add to hybridoma culture media as a 5% (v/v) supplement. Use of this product eliminates the need for feeder cells in these types of cultures.

Features

  • Increased efficiency in hybridoma production
  • No time-consuming preparation of feeder cells
  • Elimination of batch variation
  • No depletion of nutrients by feeder cells
  • Screened for mycoplasma contamination

Applications

  • Hybridoma cloning

Contents

  • BriClone Hybridoma Cloning Additive, Sample, 25ml

Publications


More citations are available upon request.