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Fluorescence Labeling Kits

FT-LNT10001-01 - Biotin PCR Labeling Kit, 100 reactions

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The Biotin PCR Labeling Core Kit provides a highly efficient, easy-to-perform and rapid labeling technology. All the reagents required for PCR labeling, with the exception of the primer, template, and materials for purification of the probe, are included. The kit is recommended for direct enzymatic labeling of DNA. The Biotin PCR labeling mix contains specially optimized Biotin-dUTP for incorporation into DNA by PCR using Taq polymerase. In PCR labeling, repeated cycles of denaturation, annealing and extension allow the amplification of a specific DNA fragment. When dTTP is partially substituted by dye-dUTP, the extension of the annealed primers with Taq polymerase generates fluorescent labeled double-stranded DNA. The resultant DNA is suited for a variety of hybridization experiments, including Southern and Northern blots, colony hybridizations and in situ hybridizations.

Features:

  • Fluorophores have excellent stability and quantum yield
  • Dye-dUTP complex has a high incorporation rate
  • Includes PCR labeling mix, Taq polymerase, PCR-grade water and 10 x buffer

 

Applications:

  • Direct enzymatic labeling of DNA in PCR
  • Other DNA labeling applications such as FISH, microarray gene expression profiling, and single molecule detection

 

Contents:

  • Biotin PCR Labeling Mix - contains 1 mM dATP, 1 mM dCTP, 1 mM dGTP, 0.5 mM dTTP, 0.5 mM Biotin-dUTP
  • Taq Polymerase, 2 units/μl
  • 10x PCR labeling buffer
  • PCR-grade water