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DNA Polymerases/SNPase

104000 - Tth Polymerase

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Tth Polymerase is a recombinant thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus thermophilus strain HB8 and expressed in E.coli. This enzyme replicates DNA at 74°C. It shows RNA-dependent DNA-polymerase activity in the presence of Mn2+ ions. Tth Polymerase has an intrinsic reverse transcription activity, but no RNAse H activity. Tth DNA polymerase can reverse transcript and amplify fragments up to 2 – 3 kb.

Features

  • Accepts modified nucleotides
  • Possesses a highly processive 5’ → 3’ polymerase activity, but has no 3’ → 5’ exonuclease activity
  • Not recommended for PCR product cloning because the error rate (3.0 × 10-5) is similar to Taq DNA polymerase
  • Thermostable

Applications

  • RT-PCR for fragments up to 2 – 3 kb
  • PCR
  • Primer extension
  • DNA labeling with modified dNTPs

Sizes


Contents

Cat# 104005

Cat# 104025

Tth Polymerase

500 units

2500 units

Tth PCR buffer (10x)

1ml

1ml x 5

RT-PCR Buffer, one-step reaction (5x)

1ml

1ml x 5

Reverse Transcription Buffer (10x)

1ml

1ml x 5

MnCl2 100 mM

1ml

1ml x 5


Literature

Publications