Home New Customer? Create Account
Member Login:

104000 - Tth Polymerase, 5 U/µl

PRICE

Starting at: $126.00

Please Choose:

Size



Add to Cart:
Tth Polymerase is a recombinant thermostable enzyme of approximately 94 kDa isolated from eubacterium Thermus thermophilus strain HB8 and expressed in E.coli. This enzyme replicates DNA at 74°C. It shows RNA-dependent DNA-polymerase activity in the presence of Mn2+ ions. Tth Polymerase has an intrinsic reverse transcription activity, but no RNAse H activity. Tth DNA polymerase can reverse transcript and amplify fragments up to 2 – 3 kb.
 

Features

  • Accepts modified nucleotides
  • Possesses a highly processive 5’ → 3’ polymerase activity, but has no 3’ → 5’ exonuclease activity
  • Not recommended for PCR product cloning because the error rate (3.0 × 10-5) is similar to Taq DNA polymerase
  • Thermostable
 

Applications

  • RT-PCR for fragments up to 2 – 3 kb
  • PCR
  • Primer extension
  • DNA labeling with modified dNTPs
 

Sizes

 
104005 (500 units)
  • Tth Polymerase, 5 U/µl, 500 units
  • Tth PCR buffer (10x), 1ml
  • RT-PCR Buffer, one-step reaction (5x), 1ml
  • Reverse Transcription Buffer (10x), 1ml
  • MnCl2 100 mM, 1ml
104025 (2500 units)
  • Tth Polymerase, 5 U/µl, 2500 units
  • Tth PCR buffer (10x), 1ml x 5
  • RT-PCR Buffer, one-step reaction (5x), 1ml x 5
  • Reverse Transcription Buffer (10x), 1ml x 5
  • MnCl2 100 mM, 1ml x 5

 

Publications