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Fluorescence PCR Labeling Kits

FT-LNT10010-01 - Atto425 PCR Labeling Kit, 10 reactions

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The Atto425 PCR Labeling Kit (10 reactions) provides a highly efficient, easy-to-perform and rapid labeling technology. All the reagents required for PCR labeling, with the exception of the primer, template, and materials for purification of the probe, are included. The kit is recommended for direct enzymatic labeling of DNA. The Atto425 PCR labeling mix contains specially optimized Atto425-dUTP for incorporation into DNA by PCR using Taq polymerase. In PCR labeling, repeated cycles of denaturation, annealing and extension allow the amplification of a specific DNA fragment. When dTTP is partially substituted by dye-dUTP, the extension of the annealed primers with Taq polymerase generates fluorescent labeled double-stranded DNA. The resultant DNA is suited for a variety of hybridization experiments, including Southern and Northern blots, colony hybridizations and in situ hybridizations.

Features

  • Abs. max = 436nm, Em. max = 484nm
  • Can be used instead of DEAC
  • Emission color = blue-green
  • Fluorophores have excellent stability and quantum yield
  • Dye-dUTP complex has high incorporation rate

Applications

  • Direct enzymatic labeling of DNA in PCR
  • Other DNA labeling applications such as FISH, microarray gene expression profiling, and single molecule detection 

Contents

  • Atto425 PCR labeling mix – contains 1 mM dATP, 1 mM dCTP, 1 mM dGTP, 0.25 mM dTTP, 0.75 mM Atto425-dUTP
  • Taq Polymerase, 1 unit/μl
  • 10x PCR labeling buffer
  • PCR-grade water

Other Sizes Available: 50 reactions

Literature


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