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EBV Assay Kits

ODZ-005 - Anti-VCA EBV IgM ELISA kit, 96 tests

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The ELISA-VIDITEST anti-VCA EBV IgM kit is intended for the diagnosis of EBV (Epstein-Barr virus)-induced or EBV-associated diseases, such as infectious mononucleosis, chronic active EBV infection, Burkitt’s lymphoma, carcinomas of Waldayer’s ring, opportunistic lymphomas (oligo- or polyclonal) and nasopharyngeal carcinoma. The kit may also be used for the overall characterization of the chronic fatigue syndrome and of immunodeficiency during which EBV is frequently activated. 

This assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific antigen that bears immunodominant epitopes of the VCA (viral capsid antigen) complex. The anti-VCA EBV antibodies, if present in the tested sera, bind to the immobilized antigens. The antibodies in complexes with antigens are later recognized by animal anti-human IgM antibodies labeled with horseradish peroxidase. The labeled antibodies are revealed by an enzymatic reaction with a chromogenic substrate. Dilution buffer “plus” contains RF sorbent to saturate IgG and rheumatoid factor. It is possible to prepare the “plus” buffer by mixing with dilution buffer and rheumatoid factor sorbent according to the instruction manual.

Features

  • Solid-phase immunoanalytical test for detection of IgM antibodies to VCA of EBV
  • Used with human serum 
  • Complete panel of EBV serological markers can be examined in single dilution of serum sample
  • Includes RF sorbent for elimination of interfering IgG antibodies
  • High sensitivity and specificity
  • Semi quantitative evaluation

Applications

  • Diagnosis of EBV-induced or EBV-associated diseases
  • Characterization of chronic fatigue syndrome and immunodeficiency related to EBV

Contents

  • ELISA break-away strips coated with specific antigens, 1 microplate
  • Calibrator, 1.3ml
  • Positive control serum, 1.3ml
  • Negative control serum, 1.3ml
  • Anti-human IgM antibodies labeled with horseradish peroxidase (Px-conjugate), 15ml
  • Wash buffer 10x concentrate, 125ml
  • Dilution buffer, 125ml
  • RF sorbent 51x concentrate, 1ml
  • Chromogenic substrate (TMB substrate), 15ml
  • Stop solution, 15ml

Literature