Our set of polyclonal and monoclonal Anti-Tet Repressor (TetR) antibodies, manufactured by MoBiTec, possess excellent binding properties and have been successfully tested for use in ELISA, Western blot and immunofluorescence assays. The immunogen is TetR-tetO (Accession no. P04483).
These antibodies can be used in the detection of Tet-Repressor (TetR), Tet-Repressor fusion protein (TetR-Fusion), tetracycline responsive transactivator (tTA) and reverse tetracycline responsive transactivator (rtTA). A TET-Repressor Protein (TETR1) is available as a positive control.
- TET01 is a polyclonal rabbit Anti-Tet Repressor antibody. It was prepared using TetR-tetO as immunogen, and has been purified on Protein G. It is provided as 200ul of purified antibody, lyophilized. After reconstitution to 200µl in distilled water, it can be further diluted 1:1000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 0.2ng; Western Blots = 0.8ng.
- TET02 is provided as 1mg of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:500 - 1:2000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 20-50pg; Western Blots = 0.8 - 1ng
- TET03 is a mouse monoclonal antibody, IgG1 Kappa. The antibody recognizes amino acid # 37-44 and is ideal for immunofluorescence studies. TET03 is provided as 50µg of purified antibody, lyophilized. After reconstitution to 100µl in distilled water, it can be further diluted 1:100 to 1:500 for immunofluorescence studies.
- TETR1 is a positive control. It is a purified 23 kDa Tet-Repressor (B) protein from E. coli transposon Tn10.
|
TET01 |
TET02 |
TET03 |
Type |
Rabbit polyclonal IgG |
monoclonal IgG1; κ mix |
monoclonal IgG1; κ |
Immunogen |
TetR-tetO |
TetR-tetO |
TetR-tetO |
Purification |
Affinity purified via Protein G columns |
Affinity purified via Protein A or G columns |
Affinity purified via Protein A or G columns |
Epitope |
-- |
TetR:
-Amino acid #84-98
-Amino acid #26-53 |
TetR:
-Amino acid #37-44 |
Reconstitution In: |
200 µl dest. H2O |
100 µl dest. H2O |
100 µl dest. H2O |
Working dilution for immunofluorescence |
n.d. |
n.d. |
1:100 - 1:500 |
Working dilution for Western Blots and ELISA |
1:1000 |
1:500 - 1:2000 |
1:1000 |
Detection limit |
0.2 ng |
20 - 50 pg |
n.d. |
Detection limit for Western Blot |
0.8 ng |
0.8 - 1.0 ng |
5 ng |
Please note: MoBiTec anti-TetR(B) antibodies were raised against TetR(B)-tetO, but are generally able to detect variants of TetR(B) as well, such as rtTA and its derivatives, whereby TET02 is the most sensitive and promising choice. However, in contrast to TetR(B), rtTA regulator proteins are required and present in cells in much smaller amounts, especially in stable cell lines. Additionally, the regulated gene and its product may downregulate these regulators further, so that it's even more obscured. Detection of rtTA and its derivatives is more difficult than that of TetR(B) and often fails at the level of Western blotting. In such cases, PCR may be used as surrogate due to its sensitivity. Nevertheless, independent references have proven the suitability of MoBiTec's anti-TetR(B) antibodies to detect rtTA. See examples below:
Therefore, MoBiTec shall not be made responsible or liable for any claims or loss arising from the failure of the anti-TetR(B) antibodies to detect rtTA and its derivatives.
Features
- Prime tool for studying tet regulatory systems in eukaryotic cells
- Suited for ELISA, Western blots and Immunofluorescence (for cells only, not for tissues or tissue slices)
- Excellent binding properties
Applications
- Detection of Tet-Repressor (TetR)
- Detection of Tet-Repressor fusion protein (TetR-Fusion)
- Detection of tetracycline responsive transactivator (tTA)
- Detection of reverse tetracycline responsive transactivator (rtTA) such as rtTA-S or rtTA-M
Products
- TET01 - Anti-Tet Repressor Antibody, polyclonal rabbit, lyophilized, 3mg
- TET02 - Anti-Tet Repressor Antibody, monoclonal IgG1, mix, lyophilized, 1mg
- TET03 - Anti-Tet Repressor Antibody, monoclonal IgG1, lyophilized, 50 µg
- TETR1 - Anti-Tet Repressor Protein (23 kDa) positive control, 1 µg
Literature
Publications
Tet Regulatory System
References with Tet-Repressor (TetR) Antibodies
- Czauderna F, Santel A, Hinz M, Fechtner M, Durieux B, Fisch G, Leenders F, Arnold W, Giese K, Klippel A, Kaufmann J (2003): Inducible shRNA expression for application in a prostate cancer mouse model. Nucleic Acids Res. 2003 Nov 1;31(21):e127.
- Leenders F, Mopert K, Schmiedeknecht A, Santel A, Czauderna F, Aleku M, Penschuck S, Dames S, Sternberger M, Rohl T, Wellmann A, Arnold W, Giese K, Kaufmann J, Klippel A (2004): PKN3 is required for malignant prostate cell growth downstream of activated PI 3-kinase. EMBO J. 2004 Aug 18;23(16):3303-13. Epub 2004 Jul 29.
- Palona I, Namba H, Mitsutake N, Starenki D, Podtcheko A, Sedliarou I, Ohtsuru A, Saenko V, Nagayama Y, Umezawa K, Yamashita S (2006): BRAFV600E promotes invasiveness of thyroid cancer cells through nuclear factor kappaB activation. Endocrinology, Dec 2006; 147: 5699 - 5707.
- Carrillo J, García-Aragoncillo E, Azorín D, Agra N, Sastre A, González-Mediero I, García-Miguel P, Pestaña A, Gallego S, Segura D, Alonso J (2007): Cholecystokinin Down-Regulation by RNA Interference Impairs Ewing Tumor Growth Clin. Cancer Res., Apr 2007; 13: 2429 - 2440.
- Weber A, Paschen SA, Heger K, Wilfling F, Frankenberg T, Bauerschmitt H, Seiffert BM, Kirschnek S, Wagner H, Häcker G (2007): BimS-induced apoptosis requires mitochondrial localization but not interaction with anti-apoptotic Bcl-2 proteins. J. Cell Biol., May 2007; 177: 625 - 636.