We are pleased to offer a unique set of polyclonal and monoclonal anti-Tet-Repressor (TetR) antibodies with excellent binding properties, manufactured by MoBiTec. The immunogen is TetR-tetO (Accession no. P04483 Pubmed). The tetracycline (tet) regulatory system is widely used for selective target gene regulation in eukaryotic cells.

These Anti-TET antibodies are suitable for the detection of:

Tet-Repressor (TetR)
Tet-Repressor fusion protein (TetR-Fusion)
tetracycline responsive transactivator (tTA)
reverse tetracycline responsive transactivator (rtTA) including derivates like rtTA-S or rtTA-M

Features:

Suited for ELISA, Western blots and immunofluorescence (not for IHC)
Excellent binding properties
Two optoins of the monoclonal antibodies are offered

TET01 is a polyclonal rabbit anti-Tet-Repressor antibody. It was prepared using TetR-tetO as immunogen, and has been purified on Protein G. It is provided as 200ul of purified antibody, lyophilized. After reconstitution to 200ul in distilled water, it can be further diluted 1:1000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 0.2ng; Western Blots = 0.8ng.

TET02 is provided as 1mg of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:500 - 1:2000 for Western blots and ELISA applications. (Monoclonal antibody TET03 is recommended for immunofluorescence studies.) This antibody has excellent binding properties to TetR. Detection limits with this antibody are as follows: ELISA = 20-50pg; Western Blots = 0.8 - 1ng

TET03 is a mouse monoclonal antibody, IgG1 Kappa. The antibody recognizes amino acid # 37-44, and is ideal for immunofluorescence studies. TET03 is provided as 50ug of purified antibody, lyophilized. After reconstitution to 100ul in distilled water, it can be further diluted 1:100 to 1:500 for immunofluorscence studies.

TETR1, is the TET Repressor Protein, positive control (23 kDa), for the detection of TETR(B)

Anti-Tet-Repressor Abs	Polyclonal rabbit IgG (TET01)	Monoclonal mouse IgG1 cocktail (TET02)	Monoclonal Mouse IgG1 (TET03)
Reconstitute in:	200ul dist. H₂O	100ul dist H₂O	100ul dist H₂O
Working dilution for Western blot/ELISA:	1:1000	1:500-1:1000	1:1000
Working dilution for immunofluorescence	n/d	n/d	1:100-1:500
Detection limit in Western blot	0.8ng	0.8-1ng	5ng
Detection limit in ELISA	0.2ng	20-50pg	n/d

Catalog #	Description	Amount	Data Sheet
TET01	anti tet-repressor, polyclonal rabbit, lyohpholized	3 mg	PDF
TET02	anti tet-repressor, monoclonal IgG1, mix lyohph.	1 mg	PDF
TET03	anti tet-repressor, monoclonal IgG1, lyoph	50 µg	PDF
TETR1	TET Repressor protein (23 kDa), positive control	1 µg	PDF

Literature:

· Pook E, Grimm S, Bonin A, Winkler T, Hillen W.(1998): Affinities of mAbs to Tet repressor complexed with operator or tetracycline suggest conformational changes associated with induction.
Eur J Biochem. 1998 Dec 15;258(3):915-22. Pubmed
Eur J Biochem. 1998 Dec 15;258(3):915-22. Pubmed
· Hillen W, Berens C.(1994): Mechanisms underlying expression of Tn10 encoded tetracycline resistance. Annu Rev Microbiol. 1994;48:345-69. Pubmed
· Gatz C.(1995): Novel inducible/repressible gene expression systems.
Methods Cell Biol. 1995;50:411-24. Pubmed
Methods Cell Biol. 1995;50:411-24. Pubmed
· Mayford M, Bach ME, Huang YY, Wang L, Hawkins RD, Kandel ER.(1996): Control of memory formation through regulated expression of a CaMKII transgene.
Science. 1996 Dec 6;274(5293):1678-83. Pubmed
Science. 1996 Dec 6;274(5293):1678-83. Pubmed
· Hwang JJ, Scuric Z, Anderson WF. (1996): Novel retroviral vector transferring a suicide gene and a selectable marker gene with enhanced gene expression by using a tetracycline-responsive expression system. J Virol. 1996 Nov;70(11):8138-41. Pubmed Free
· Gossen M, Freundlieb S, Bender G, Muller G, Hillen W, Bujard H. (1996): Transcriptional activation by tetracyclines in mammalian cells. Science. 1995 Jun 23;268(5218):1766-9. Pubmed
· Hinrichs W, Kisker C, Duvel M, Muller A, Tovar K, Hillen W, Saenger W (1994): Structure of the Tet repressor-tetracycline complex and regulation of antibiotic resistance. Science. 1994 Apr 15;264(5157):418-20. Pubmed

Anti-Tet-Repressor Antibodies Information Sheet