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Borrelia Assay Kits

ODZ-173 - Anti-Borrelia recombinant IgM (CSF) ELISA kit, 96 tests

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The ELISA-VIDITEST anti-Borrelia recombinant IgM (CSF) kit is intended for the detection of IgM antibodies to the pathogenic borrelia strains (B. afzelii, B. garinii and B. burgdorferi sensu stricto) in human serum or plasma, cerebrospinal fluid and synovial fluid. The detection of antibodies is one of the laboratory tests that help to diagnose Lyme disease.

This assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with the mixture of recombinant antigens. Anti-borrelia antibodies in serum samples bind to the immobilized antigens. The serum antibodies that do not bind are washed away and those that formed complexes with the antigens are later recognized by animal anti-human IgG antibodies labeled with horseradish peroxidase. The presence of labeled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative sera do not react. A mild change in color, if present, may be attributed to the reaction background.

Features

  • Solid-phase immune-analytical test the detection of IgM antibodies
  • Used with human serum or plasma, cerebrospinal fluid and synovial fluid
  • High sensitivity/specificity (99% / 97%)
  • Highly specific recombinant antigens
  • Qualitative evaluation

Applications

  • Intrathecal IgM synthesis determination
  • Antibody determination in serum, cerebrospinal fluid and synovial fluid
  • Detection of Lyme disease

Contents

  • ELISA break-away strips coated with specific recombinant antigens, 1 microplate
  • Control, 1.3ml
  • Standard B, 1.3ml
  • Standard 2, 1.3ml
  • Standard 1, 1.3ml
  • Standard E, 1.3ml
  • Anti-human IgM antibodies labeled with horseradish peroxidase (Px-conjugate), 15ml
  • Wash buffer concentrate, 10x, 125ml
  • Dilution buffer, 100ml
  • Chromogenic substrate (TMB substrate), 15ml
  • Stop solution, 15ml

Literature