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3D Transfection

TF20000 - 3D-Fect for 3D Scaffolds


Starting at: $155.00

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3D-Fect™ is a 3D Transfection Reagent specifically developed to directly transfect cells cultured in 3D scaffolds. It is suitable for all types of scaffolds and cells. 3D matrices add a third dimension to the cells’ environment and can induce significant differences in cellular phenotype and behavior. Scaffold-based 3D matrices activated with 3D-Fect™/DNA complexes are colonized by cells which are subsequently transfected in situ and in a more natural environment. These transfections can be studied in tissue engineering and regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation, colonization, neurite growth and other areas.

Figure 1: 3D-Fect allows transfection of a variety of cells (cell lines and primary cells) into 3D-scaffolds: Collagen-based and collagen-derived scaffolds were pre-loaded with complexes formed by 3D-Fect™ (ratio 4:1) and 1 μg of either pVectOZ-GFP (green pictures) or RFP plasmids (red pictures). Cells were then seeded and observed under fluorescence microscopy after 48 h.

Figure 2: Alkaline phosphatase secretion of cells transfected into 3D Scaffold: Collagen-derived scaffolds were pre-loaded with complexes formed by 1 μg of pVectOZ-SEAP and 4 μl of 3D-Fect reagent. Cells were then seeded and secreted alkaline phosphatase (SEAP) concentration was measured after 48 h.


  • Highly efficient
  • For 3D Scaffolds
  • Compatible with all types of nucleic acids 
  • Can be used with cell lines and primary cells
  • Completely biodegradable
  • Can be used for long term protein expression
  • Serum Compatible


  • DNA transfection of cells growing in 3D-scaffolds


  • TF20250 – 3D-Fect™ 3D transfection reagent, 250 µl (65-125 transfections)
  • TF20500 – 3D-Fect™ 3D transfection reagent, 500 µl (125-250 transfections)
  • TF21000 – 3D-Fect™ 3D transfection reagent, 1ml (250-500 transfections)