Western Blotting

This is the most convenient chemiluminescent Western Blot Detection System available. It consists of a single reagent in a convenient spray bottle format. You no longer have to mix reagent A and reagent B before processing your Western Blots. Just spray and expose!

Features:

• Single reagent spray-on format
• Rapid reaction time - specific protein detection in as little as 1 minute
• High sensitivity - detect as little as 1-2pg of antigen
• High resolution - West-one generates high contrast signal
• Stable for more than one year

Protocol:

1. Just spray WEST-one on the membrane (2 clicks)
2. Incubate for just 1 minute
3. Drain off excess detection reagent
4. Wrap membranes and place in cassette
5. Place an X-ray film on the membrane and expose

Sensitivity Test

Lane 1: 20ng BSA; Lane 2: 2ng BSA; Lane 3: 200pg BSA; Lane 4: 20pg BSA

Packaging: 100ml spray bottle; 500µl per click; 2 clicks per standard blot. Reagent contains luminol, H2O2, enhancer, and stabilizer.