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VeroFect Vero Cell Transfection

VF60000 - VeroFect Vero Cell Transfection Reagent

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Starting at: $163.00

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VeroFect is a specific reagent dedicated to the transfection of Vero cell lines (African Green Monkey kidney epithelial cells). VeroFect is based on TEE Technology (Triggered Endosomal Escape), a mechanism that combines and exploits the properties of cationic lipids and polymers to achieve extremely efficient DNA delivery into cells. The association of nucleic acids with the VeroFect reagent results in the tight compaction and protection of the DNA. The use of Verofect allows efficient destabilization of the endosomal membrane and the release of large DNA amounts in the cytosol.


Figure 1: Protocol. Tee-Technology combines and exploits the properties of cationic lipids and polymers to achieve an extremely efficient DNA delivery into cells. 


Figure 2: High transgene expression after VeroFect-mediated transfection of Vero cells. Vero cells (5x104) were transfected with 1 μg/well of pEGFP plasmid and 2 μl of VeroFect in a 24-well plate. GFP expression was observed 48h after transfection by fluorescence microscopy.


Figure 3: VeroFect outperforms competitors. Vero cells (5x104) were transfected with 1μg/well of pEGFP or pLacZ plasmid and 2 μL of VeroFect in a 24-well plate. GFP expression was monitored 48 h after transfection by flow cytometry.

Features

  • Specialized for Vero cell lines
  • Highly efficient 
  • Simple, rapid protocol
  • Compatible with and without serum-containing culture media
  • Non-toxic and economical  

Applications

  • Transfection of Vero cells

Sizes

  • VF60250 – VeroFect Transfection Reagent, 250 µl (125 transfections)
  • VF60500 - VeroFect Transfection Reagent, 500 µl (250 transfections)
  • VF61000 - VeroFect Transfection Reagent, 1ml (500 transfections)