This system combines the benefits of the tightly regulated and strong T7 RNA polymerase expression system and alkaline protease free Bacillus megaterium. The system is based on two parallel replicating plasmids:pT7-RNAP and pPT7. For your convenience we offer B. megaterium protoplasts pretransformed with pT7-RNAP, so that you just have to insert your gene of interest into pPT7 and transform the pretransformed protoplasts with this plasmid.


