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Blockers

110 000 - The Blocking Solution

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The Blocking Solution is designed for saturating free binding sites on plastic surfaces or other protein binding surfaces and for minimizing nonspecific binding to surfaces. It is based on highly purified casein that has been chemically modified for maximum adsorption efficiency and a dense blocking layer. By incubating a surface with The Blocking Solution, such as a microtiter plate or western blotting membrane, free spaces on the surface are saturated by the components of this blocker. This minimizes unwanted binding of the analyte or the detection antibody to the surface, avoiding background and improving sensitivity of the assay. The Blocking Solution is well suited for assays that require high reproducibility and low coefficients of variation, such as assays for pre-clinical and clinical studies, production of high quality diagnostics or measurement of precious specimens.

Features

  • Chemically modified casein-based blocker
  • Saturates free binding sites on surfaces 
  • Reduces nonspecific binding to surfaces and reduces background
  • Ideal for assays that require high reproducibility and low coefficients of variation
  • Improves sensitivity of the assay

Applications

  • ELISA
  • Western blotting
  • Protein Arrays
  • Immuno-PCR
  • Immunohistochemistry
  • Enzyme immunoassay
  • Radioimmunoassay

Sizes

  • 110 050 – The Blocking Solution, 50ml
  • 110 125 – The Blocking Solution, 125ml
  • 110 500 – The Blocking Solution, 500ml

Literature