DNA Polymerases/SNPase

SuperHotTaq Description

SuperHotTaq (Hot Start) DNA Polymerase is an optimized mixture of Taq Polymerase and Anti-Taq monoclonal antibodies. Antibodies block polymerase activity during set-up of the PCR reactions at ambient temperature (20-22°C). The inhibition of Taq DNA polymerase is completely reversed when the temperature is above 70°C. The PCR products obtained with SuperHOT Tq are free of non-specific products and from primer-dimers.

SuperHotTaq Features

• Reliable and reproducible quantification in qPCR
• Perfect for Realtime PCR
• Especially for diagnostic purposes
• Reaction set-up at room temperature
• Activation of enzyme during first heating
• No change or optimization of protocol necessary
• High specifity, reduced primer mismatch or dimers

SuperHotTaq Applications

• Hotstart PCR
• Realtime PCR
• Amplification of complex genomic and cDNA templates
• Multiplex PCR
• High specifity PCR

CONCENTRATION
5 Units//µl

STORAGE
-20°C

STORAGE BUFFER
10 mM Tris-HCI (pH 7.0)
50 mM KCl
0.1 mM EDTA
50% glycerol