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Protein Gel/Blot Detection

PR-SB01-01 - SpeedBlot (His) Western Blot Stain



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SpeedBlot (His) Western Blot Stain

Step 1
Start: Pour SpeedBlot (His) into suitable container
Step 2
1 min: Submerge membrane in SpeedBlot (His)
Step 3
10 min: Incubate until signal is clearly visible (30-90 min)
Step 4
60 min: Stained poly-His proteins on the membrane

  • Fastest Western ever; results in 30-90 min
  • Specific staining of His-tagged proteins
  • Sensitive to sub-picomol amounts of protein
  • Simple incubation, no buffer exchange, etc
  • Economic, no readout equipment required
  • Visually detectable result
  • Quantitative and permanent signal
  • Works with C-/N-terminal tags

SpeedBlot (His) is a ready-to-use reagent for the detection of poly-histidine tagged proteins in blot assays. This reagent is equally suitable for Western Blot assays. The detection reagent is made of an optimized anti-histidine antibody gold nanoparticle conjugate and is reactive against C- and N- terminal tags. The protein of interest is specifically stained in pink directly on the blot. It contains the Qiagen Penta-His Antibody which is covered by patents exclusively licensed to Qiagen GmbH, Germany.

Instant detection of poly-histidine tagged proteins on nitrocellulose membrane with SpeedBlot (His) is easy and does not require relevant hands-on time. Even 0.1pmol detection limits can be achieved with this highly sensitive detection reagent. This is in the range of conventional ECL detection. 

Comparison of His-tag detection methods

Comparison of His-tag detection methods