DNA Polymerases/SNPase

SNPase DNA Polymerase

SNPase is a Taq DNA Polymerase with an N-terminal deletion and proprietary amino acid substitutions introduced into the active center of the enzyme. This modification causes a dramatic increase in sensitivity to mismatches at the 3'-end of the primer. Consequently, non perfect annealing of the primers does not result in non specific amplicon formation. These properties make SNPase ideal for SNP detection by allele specific PCR and micro sequencing.

SNPase FEATURES

• High sensitivity to mismatches at the 3'-end of primers
• High fidelity of dNTP and ddNTP incorporation
• Lack of 5'- and 3'- exonuclease activity
• High specificity, low level of background PCR

SNPase APPLICATIONS

• SNP-genotyping, allele-specific PCR, allele specific primer extension, mini sequencing
• Highly specific PCR, including multiplex PCR
• Highest fidelity PCR (for fragments below 500bp)
• Real Time PCR with intercalating dyes

SNPase LIMITATIONS

• SNPase cannot be used in TaqMan assay due to lack of 5'-exo activity
• SNPase cannot be used for effective PCR of templates longer than 500bp