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3D Transfection

STN50000 - si3D-FectIN


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si3D-FectIN is a 3D transfection reagent specifically designed and developed for silencing gene expression in cells cultured in gels (or hydrogels).

3D matrices not only add a third dimension to the cells' environment, they also allow creating significant differences in cellular phenotype and behavior. In this way, 3D matrices bearing complexes formed with si3D-FectIN reagent and siRNA are colonized by cells to be transfected in a more natural environment. 
  • Highly efficient for gene silencing in 3D matrices
  • Ideal for any gel and hydrogel
  • Dedicated to short nucleic acid sequences (siRNA, miRNA...)
  • Long term gene silencing
  • Universal (primary cells and cell lines)
  • Serum Compatible
Perfect for all transfection applications in 3D hydrogels such as collagen, hyaluronic acid, PEG, fibrin, laminin:
  • angiogenesis
  • tube and acini formation
  • colonization
  • neurite growth
  • tissue engineering
  • tissue regeneration
  • tumor invasion
  • neural differentiation
  • cellular polarization
  • tissue formation
RECOMMENDED FOR: Gene silencing of cells growing in 3D-hydrogels

siRNA Transfection in hydrogels with si3D-FectIN Reagent
Figure 1: si3D-FectIN allows efficient siRNA transfection in hydrogels: Collagen-derived hydrogels were loaded with 50nM of fluorescently labelled siRNA complexed to 4µL of si3D-FectIN transfection reagent. Transfection efficiency of COS-7 and NIH-3T3 cells were observed after 48h by fluorescence microscopy.