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DNA Polymerases/SNPase

108000 - SD Polymerase

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SD POLYMERASE

SD Polymerase is a novel artificial thermostable polymerase providing a strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity as Phi29 or Bst Polymerase, which are active below 68 °C only, SD Polymerase is stable up to 93 °C. This allows running isothermal amplification like LAMP with initial heating-up for increased specificity. 


SD Polymerase has 5’ – 3’ polymerase and 5’ – 3’ strand displacement activities. It does not have any exonuclease activity. The enzyme does 3’-A-overhangs. SD Polymerase is also available as a HotStart version.

 

SD Polymerase is used in our WGA-Kit for whole genome amplification


Features:

· Available in high and low concentrations and as a HotStart version

· Higher yields, speed and efficiency compared to Taq Polymerase

· Suitable for amplification of short (100 bp) and long (20 – 30 kb) DNA fragments

· Suitable for simple (e.g. plasmids) and complex (e.g. genomic DNA) templates

 

Applications:

· Suitable for different PCR methods, also long-range PCR

· PCDR (Polymerase Chain Displacement Reaction)

· Strand displacement like LAMP (Loop-mediated isothermal amplification)



Kit Contents

SD Polymerase
Catalog Number
200 units
108702
1000 units
108710
1000 units
108800
5000 units
108850

SD Polymerase*
Reaction Buffer (10x)
1.8ml2 x 1.8ml2 x 1.8ml5 x 1.8ml
MgCl2 100mM1ml1ml1ml3 x 1ml
Datasheet 1111
*SD Polymerase Reaction Buffer, complete (10x), pH 8.9, with Tween-20 and BSA; please add MgCl2 to 3-3.5mM end concentration just before use; adding MgCl2 to the 10x buffer may precipitate at 4C storage




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