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DNA Polymerases/SNPase

108000 - SD Polymerase

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SD Polymerase is a novel artificial thermostable polymerase with a strong strand displacement activity. Unlike natural enzymes with strong strand displacement activity such as Phi29 or Bst Polymerase, which are active below 68°C only, SD Polymerase is stable up to 93°C. This enables running isothermal amplification such as LAMP with initial heating for increased specificity. SD Polymerase has 5’ – 3’ polymerase and 5’ – 3’ strand displacement activities. It does not have any exonuclease activity. The enzyme does 3’-A-overhangs. 

SD Polymerase is offered as 10 U/µl, 50 U/µl, and as a HotStart version. SD Polymerase is also used in Bioron's WGA kit for whole genome amplification.

Features

  • Stable up to 93°C
  • Available in high and low concentrations and as a HotStart version
  • Higher yields, speed and efficiency compared to Taq Polymerase
  • Suitable for amplification of short (100 bp) and long (20 – 30 kb) DNA fragments
  • Suitable for simple (e.g. plasmids) and complex (e.g. genomic DNA) templates

Applications

  • Various PCR methods including long-range PCR
  • PCDR (Polymerase Chain Displacement Reaction)
  • Strand displacement including LAMP (Loop-mediated isothermal amplification)

Formats


SD Polymerase

Contents

108702

108710

108800

108850

SD Polymerase

200 units, 10 U/µl

1000 units, 10 U/µl

1000 units, 50 U/µl

5000 units, 50 U/µl

SD Polymerase Reaction Buffer Incomplete (10x)

1.8ml

1.8ml x 2

1.8ml

1.8ml x 2

MgCl2 100 mM

1ml

1ml

1ml

1ml


SD Polymerase HotStart

Contents

108902

108910

SD Polymerase HotStart

200 units, 10U/µl

1000 units, 10U/µl

SD Polymerase Reaction Buffer Incomplete (10x)

1.8ml

1.8ml x 2

MgCl2 100 mM

1ml

1ml


Literature

Publications