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OG179 - pSF-RecA-RrnB

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This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Vector: pSF-RecA-RrnB

Product Code: OG179

Quantity Provided: 5µg

Size (bp): 3601

Bacterial Antibiotic Selection: Kanamycin

Origin and Compatibility: pUC high copy, derived from pBR322   

Copy Number: 500-700 copies per cell

Promoter: RecA bacterial promoter with the LexA repressor binding site deleted.

Purpose: Expression in bacterial cells. The RecA promoter is a very strong constitutive promoter. It is approximately 650 x stronger than the constitutive AraBAD promoter that we also sell. The RecA promoter is normally  regulated by the repressor LexA. In this promoter this binding site has been ablated to enable constitutive expression. This plasmid is used when the T7 terminator and the SV40 poly-adenylation sites that are found within most of our vectors are not required.     

Transcription Termination: This plasmid contains only a bacterial terminator after the multiple cloning site. The T7 and mammalian termiators have been deleted in this plasmid. 

Inserting a Gene: Each restriction site in this vector has a purpose and allows the insertion of specific pre-designed DNA sequences. To insert a gene, we recommend using any sites between (and including) the HindIII and XbaI sites. By doing this you will be able to insert most of our other products upstream, or downstream, of your gene. Click here to read more about the purpose of each restriction site. 

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Quality Validation: This vector has been demonstrated to express reporter genes to high levels under the RecA promoter in Escherichia.coli. The start codon of these reporters was positioned within the NcoI site and the stop codon was positioned within the XbaI site.  

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Genetic Modifications to standard parts:

  • RecA promoter – The LexA binding site has been ablated in this promoter to provide constitutive expression.  
  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFast system and to retain a high level of antibiotic resistance. It has been validated in E.coli.

Restriction site notes:

  • Bgl2, KpnI, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, start codon, origin, and KanR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.