Home New Customer? Create Account
Member Login:

GFP

OG241 - pSF-pA-PromMCS-daGFP

PRICE

Starting at: $307.00

Please Choose:

Shipping Option



Enter Quantity:



This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Vector: pSF-PromMCS-daGFP

Product Code: OG241

Quantity Provided: 5µg

Size (bp): 4540 bp

Bacterial Antibiotic Selection: Kanamycin

Origin and Compatibility: pUC high copy, derived from pBR322   

Copy Number: 500-700 copies per cell

Promoter: Not applicable

Purpose: This plasmid contains a multiple cloning site in the promoter position designed to allow you to insert your own promoter to drive the expression of the reporter gene. In this plasmid it is upstream of the green fluorescent reporter gene daGFP. The promoter multiple cloning site extends for the SalI restriction ite to the BstBI restriction site. Downstream sites have other functions in our plasmid system, for example, adding n-terminal peptide tags.

Poly A signals: The promoter multiple cloning site region in this plasmid contains a synthetic poly-adenylation site. This reduces the level of background transcription from the plasmid. However, if you intend to insert this region into a lentivirus or retrovirus, the polyA signal will reduce the virus titre because of premature genome termination. We also have a variant of this plasmid without the polyA signal that can be used for this purpose if it is required.  

daGFP: This vector encodes a green fluorescent protein called daGFP. This protein can be used in the same way as normal GFP using argon laser based or UV based excitation apparatus to allow the detection of fluorescence. The protein has a peak excitation of 510nm and a peak emission of 521nm. The gene encoding the protein is sold free of intellectual property restrictions.

Transcription Termination: This plasmid contains three alternative transcription terminators for mammalian, bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Genetic Modifications to standard parts:

  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFast system and to retain a high level of antibiotic resistance. It has been validated in E.coli.
  • daGFP – The daGFP reporter gene has been modified to remove PciI and BseRI restriction sites.  

Restriction site notes:

  • Bgl2, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, origin, and KanR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.