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OG34 - pSF-LacUV5 (unreg)


Starting at: $307.00

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This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Product Code: OG34

Size (bp): 3845

Bacterial Antibiotic Selection:

Origin and Compatibility:
 pUC high copy, derived from pBR322   

Copy Number:
 500-700 copies per cell

LacUV5 unregulated promoter

 This vector can be used for the expression of proteins in bacteria using a constitutive promoter. It does not require induction. This construct is designed to induce intermediate levels of transcription inEscherichia coli cells, with the RecA (ΔLexA binding) promoter that we sell being stronger, and the AraBAD (unregulated) promoter being weaker than this promoter. Transcription terminates at the rrnG terminator that is found in all SnapFastTM vectors.

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Quality Validation: This vector has been demonstrated to express reporter genes to intermediate levels in E. coli under the LacUV5 unregulated promoter. The start codon of these reporters was positioned within the NcoI site and the stop codon was positioned within the XbaI site.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Genetic Modifications to standard parts:

  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFastTM system and to retain a high level of antibiotic resistance. It has been validated in E.coli.

Restriction site notes:

  • Bgl2, KpnI, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, start codon, origin, and KanR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.