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Ampicillin Selection Plasmids

OG3 - pSF-CMV-deltaNcol

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This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Product Code: OG3

Size (bp): 4304

Bacterial Antibiotic Selection: Ampicillin

Origin and Compatibility: pUC high copy, derived from pBR322 Copy Number: 500-700 copies per cell

Promoter: Cytomegalovirus (CMV) immediate early promoter

Purpose: This plasmid yields high levels of protein expression from the CMV promoter in mammalian cells, but it contains no start codon in the MCS, unlike most of our other plasmids. This allows you to insert genes into the downstream restriction sites and use your own genes start codon if required, thereby preventing premature translation initiation at the start codon that was within the NcoI site.

Transcription Termination: This plasmid contains three alternative transcription terminators for mammalian, bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Inserting a Gene: Each restriction site in this vector has a purpose and allows the insertion of specific pre-designed DNA sequences. To insert a gene, we recommend using any sites between (and including) the HindIII and XbaI sites. By doing this you will be able to insert most of our other products upstream, or downstream, of your gene. Click here to read more about the purpose of each restriction site.

Quality Validation: This plasmid has been demonstrated to express reporter genes to high levels under the CMV promoter. Expression was validated in the A549 lung carcinoma cell line.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Genetic Modifications to standard parts:

  • CMV promoter – an NcoI site has been ablated in the CMV promoter. This change does not reduce expression.
  • AmpR cassette – This AmpR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFast system and to retain a high level of antibiotic resistance. It has been validated in E.coli.


Restriction site notes:

  • Bgl2, SwaI and PmeI each cut the plasmid at two sites positioned to flank the promoter, origin, and AmpR, respectively.
  • BsgI and BseRI recognise non-palindromic DNA sequences and cleave upstream of their recognition sites, within the stop codon that is found inside the XbaI restriction site.