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Mammalian

OG57 - pSF-CMV-COOH-EKT-CMyc2

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This plasmid is compatible with all of the other DNA sequences available on this site, which are all sold in the same backbone. This flexibility allows you to create any vector you require, with a variety of functions, simply by compiling the relevant sections from our product range.

Product Code: OG57

Size (bp):
 4272

Bacterial Antibiotic Selection:
 Kanamycin

Origin and Compatibility:
pUC high copy, derived from pBR322   

Copy Number:
 500-700 copies per cell

Promoter: 
Cytomegalovirus (CMV) immediate early promoter

Purpose: 
This vector adds a C-Myc tag that allows protein detection or purification using antibodies against the peptide. The C-Myc tag will be positioned at the 3’ end (C-terminus) of a gene (expressed protein) in the primary MCS (NotI-XbaI). For reference, the coding sequence of the C-Myc tag is one base pair out of frame with the TAG stop codon that is found within the upstream XbaI site in pSF-CMV. The C-Myc tag coding sequence is EQKLISEEDL. There is an enterokinase cleavage site immediately upstream of this tag (DDDDK) that can be used to remove the tag from a purified protein.

Transcription Termination: This plasmid contains three alternative transcription terminators for mammalian, bacterial and bacteriophage (T7) expression. This means that only the promoter needs to be changed to alter the expression system you are using. We sell multiple promoters that can be used in each of these systems. The presence of each terminator does not reduce expression in the alternative systems.

Inserting a Gene: Each restriction site in this vector has a purpose and allows the insertion of specific pre-designed DNA sequences. To insert a gene, we recommend using any sites between (and including) the HindIII and XbaI sites. By doing this you will be able to insert most of our other products upstream, or downstream, of your gene. Click here to read more about the purpose of each restriction site.

Intellectual Property Status: According to our IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.

Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through our website.

Genetic Modifications to standard parts:

  • CMV promoter – an NcoI site has been ablated in the CMV promoter. This change does not reduce expression.
  • KanR cassette – This KanR resistance region is not a wild type sequence, it has been modified to remove all restriction sites that conflict with the SnapFast system and to retain a high level of antibiotic resistance. It has been validated in E.coli.

Restriction site notes:

  • Bgl2, KpnI, SwaI and PmeI each cut the vector at two sites positioned to flank the promoter, start codon, origin, and KanR, respectively.