Home New Customer? Create Account
Member Login:

Antibody Purification

PR-PGK002 - Protein G Starter Kit - Immunoglobulin Purification - 2 columns

PRICE

Starting at: $564.00

Please Choose:

Shipping Option



Enter Quantity:

Protein G Starter Kit - Immunoglobulin Purification - 2 columns

Protein A and Protein G are bacterial proteins with high specific binding to the Fc (non-antigen binding) domain of most classes of antibodies. Protein A and Protein G are ideal for affinity purification of immunoglobulins or for the separation of Fc from Fab fragments.

The binding characteristics of Protein A and Protein G differ:

Protein A has a broad species reactivity. It binds well to IgG from human, rabbit cow and guinea pig but weakly to mouse monoclonal antibodies.

Protein G has a broader species reactivity. It shows strong binding to IgG from mouse (including monoclonal antibodies), rat and goat.

These complete kits have everything you need for antibody purification including the purification columns, buffers, ultrafiltration spin columns and tubes.

Starter Kit Features

  • Purification of monoclonal antibodies (mouse and rat)
  • Selective purification of antibody fragments
  • Purification or removal of polyclonal IgG from serum
  • Separation of Ig subclasses using a stepwise pH gradient.
  • Protein A for Ig from human (IgM, IgA, IgE, IgG1, IgG2, IgG4), mouse, cat, dog, rabbit, pig, and guinea pig
  • Protein G for Ig from human (IgG1, IgG2, IgG3, IgG4), mouse, cow, goat, horse, pig, rabbit, rat and sheep

IMMUNOGLOBULIN BINDING PROPERTIES OF PROTEIN A AND PROTEIN G

Immunoglobulin/Fragment

Protein A

Protein G

Human IgG1

++

++

Human IgG2

++

++

Human IgG3

-

++

Human IgG4

++

++

Human IgA

+

-

Human IgD

+

-

Human IgE

+

-

Human IgM

+

-

Mouse IgG1

+

+

Mouse IgG

++

++

Mouse IgG2b

++

++

Mouse IgG3

+

++

Mouse IgM

+/-

-

Rat IgG

++

++

Rat IgG1

+/-

++

Rat IgG2a

+/-

++

Rat IgG2b

+/-

+

Rat IgG2c

+/-

+

Rat IgM

+/-

-

Rabbit IgG

++

++

Hamster IgG

+

++

Guinea Pig IgG

++

+

Bovine IgG

+

+

Sheep IgG

+/-

+

Goat IgG

+/-

+

Pig IgG

++

++

Chicken IgG

-

+/-

Human Fab

+

+

Human (Fab')2

+

+

Human scFv

+

-

Human Fc

+

+

Human Kappa Light Chains

-

-

Human Lambda Light Chains

-

-