Protein G originating from Streptococci is a monomeric protein with a molecular weight of about 50,000 kDa. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from Mouse, Rabbit, Goat and Human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins.

Recombinant Protein-G used in Adar’s Protein G beads is a genetically engineered form of Protein-G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads, using Adar Biotech's TargetLock chemistry, in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP).

Protein G Beads Specifications

Matrix: Sepharose^TM CL-4B
Coupling method: TargetLock.
Protein G density: 3-4 mg/ml of gel
Binding capacity: ~4-8 mg rabbit IgG per ml of beads
Mean bead size: 40 -165 μm
Bead structure: Highly cross-linked spherical agarose, 4%
Max back pressure: 0.3 MPa, 3 bar
Max. flow rates: 4 ml/min/cm²
Recommended flow rate: 1-3 ml/min/cm²
Stability of the matrix: pH 2-11.
Storage: 4°C in PBS pH 7.4 added with NaN3 0.1% (w/v) as a preservative.