pLUG« TA Cloning Vectors are designed for convenient and direct PCR cloning with improved blue/white selection. DCSTM technology (Dual alpha-Complementation System) eliminates false positive colonies with a parental plasmid or with a re-circularizedáTA-vector after loss of the T-overhang.
ápLUG« TA Cloning Vectors contain several engineered restriction enzyme recognition sites around the TA cloning site allowing easy restriction analysis of recombinant plasmids and re-cloning to another vector.
- High cloning efficiency
- High percenatge of true white colonies with DCSTM technology
- Dependable blue/white colony selection with DCSTM technology
- Rapid ligation protocol (10-15 minutes)
- M13 forward/reverse priming sites for sequencing
- pLUG« TA Cloning Vector, 20μl
- 5X ligation buffer, 100μl
- T4 DNA ligase, 20μl
- Distilled water, 1ml