Fusion Protein Purification

Phenylboronate is a very stable matrix with affinity to beta-lactamase, Igase and peroxidase. Columns with immobilized phenylboronate specifically bind beta-lactamase in loading buffer; the enzyme can be washed out off the columns by elution buffer. The columns are particularly useful for the isolation of fusion proteins containing beta-lactamase, such as produced e.g. with our PheBo Fusion Protein Cloning System.

COLUMN PROPERTIES:

• stable ligand immobilization
• high coupling capacity
• high mechanical stability
• improved flow characteristics
• re-usable

About 10 mmole phenylboronate are immobilized per ml sepharose beads. Following the general protocol, 1 ml of phenylboronate sepharose binds at least 3 mg beta-lactamase.