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CMV-based Constitutive Expression Vectors

VS-EXC00100-01 - phCMV1 CMV-based constitutive expression vector (native form), Kit

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The phCMV series of vectors are designed to achieve significantly higher expression levels than traditional human cytomegalovirus (CMV) promoter-based constitutive expression vectors. This makes the phCMV vectors ideal tools for routine protein expression studies as well as animal injection experiments.
The phCMV vectors consistently deliver superior expression levels when compared with other commercially available CMV promoter-based expression vectors. It is not unusual to obtain protein expression levels from phCMV that are severalfold higher than those obtained from other popularly used vectors. In addition, because phCMV vectors use the Kan/Neo gene for selection in both bacteria and cultured cell lines, they offer smaller vector sizes and improved transfection efficiency.

In summary, the three phCMV vectors offer the following benefits:comparison

  • Maximized high-level expression with optimized CMV promoter
  • G418 resistance gene for the selection of stable cell lines
  • Optional N- or C-terminal HA fusion tags for simplified protein detection and purification with
    anti-HA antibodies and affinity resins
  • Extensive multiple cloning region for convenient and easy cloning
  • Small vector sizes for efficient transfection

FEATUREFUNCTION
Modified human CMV immediate-early promotor/enhancer/intronHigh-level expression promoter in vitro & in vivo
T7 promoter priming site in phCMV, phCMV3For sequencing of the inserted DNA fragment
SV40 polyadenylation signalEfficient transcription termination and polyadenylation of mRNA
SV40 promoterHigh-level expression of the neomycin resistance gene for selection of stably transfected cells
Kanamycin resistance geneEfficient selection of vector in E. coli cells
pUC originHigh copy number replication of vector in E. coli cells
Multiple cloning site and HA tagsFor cloning a gene into vector wild type, N-terminal HA, or C-terminal HA tag 

Available Kits and Contents

KITCONTENTS
phCMV1 for encoding rproteins in their native from without any tags25μg supercoiled phCMV1 vector; 10μg phCMV1/CAT positive control vector
phCMV2 for encoding rproteins in their native from with N-terminal HA tag25μg supercoiled phCMV2 vector; 10μg phCMV2/CAT positive control vector
phCMV3 for encoding rproteins in their native from with C-terminal HA tag25μg supercoiled phCMV3 vector; 10μg phCMV3/CAT positive control vector