DNA Polymerases/SNPase

Bacteriophage T7 DNA is isolated from an infected E. coli strain. DNA is linear, 39937 bp long. Some restriction enzymes have no recognition sequences on lambda DNA, therefore these enzymes are to be analyzed on an alternative DNA substrate. T7 DNA is the most popular "alternative" to Lambda DNA substrate for analysing restriction endonucleases. The complete nucleotide sequence has been determined (1).

Click here for complete sequence

Storage Buffer:
10mM Tris-HCl (pH 7.8), 10mM NaCl, 1mM EDTA.

Storage:
Store at -20°C.

References:
1.Dunn, J.J. and Studier, F.W. (1983) Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements, J. Mol. Biol. 166 (4), 477-535.