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Mis12 Complex

VS-MSV00061 - pG-20-hNsl1; 15ug

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Expression of EGFP Fusion Proteins for the Investigation of the Human Mis12 Complex

Features
  • Ready-to-use vectors for live cell imaging
  • Large selection of genes
  • Sequence-documented vectors
  • Applications include localization, binding, and protein-protein interaction studies
  • Experimentally approved
Description
At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic checkpoint. The Mis12 complex is a link between the inner and outer kinetochore. We offer human Mis12 complex proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N- or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g. for vector pG-22-hMis12 the linker length is 22 amino acids).

Schematic view of the Mis12 Complex

Application
The Mis12 complex EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
  • localization
  • binding
  • dynamics, and, when a second color is available
  • protein-protein interactions, and
  • protein-protein proximities
applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H

Please note:
For FRET analysis we suggest fusion proteins with short linker.