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Mitotic Checkpoint

VS-MCV00081 - pG-20-hMad1; 15ug

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Expression of EGFP Fusion Proteins for the Investigation of the Mitotic Checkpoint

Features
  • Ready-to-use vectors for live cell imaging
  • Large selection of genes
  • Sequence-documented vectors
  • Applications include localization, binding, and protein-protein interaction studies
  • Experimentally approved
Description
At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic checkpoint. We offer a large number of human mitotic check point proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with the N- or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g. for vector pG-20-hCdc20 the linker length is 20 amino acids).

Three dimensional kinetochore model by Doring (2012)

Application
The mitotic checkpoint EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
  • localization
  • binding
  • dynamics, and, when a second color is available
  • protein-protein interactions and
  • protein-protein proximities
applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.

Please note:
For FRET analysis we suggest fusion proteins with short linker