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Inner Kinetochore Fusion Proteins

VS-KIVO0181 - pG-20-hCenpO; 15ug

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Expression of EFGP Fusion Proteins for the Investigation of the Kinetochore Functions

Features:
  • Ready-to-use vectors for live cell imaging
  • Large selection of genes
  • Sequence-documented vectors
  • Applications include localization, binding, and protein-protein interaction studies
  • Experimentally approved
Description:
At each centromere, a multi-protein complex binds named the kinetochore. The inner kinetochore directly binds to the centromeric chromatin during the whole cell cycle. During mitosis, further proteins of the outer kinetochore bind to the inner kinetochore and establish the stable link between the centromeric chromatin and the microtubuli. This mechanical connection is regulated by proteins of the mitotic checkpoint. We offer a large number of expression vectors coding for human kinetochore proteins in fusion with EGFP. Most of the fusion proteins can be expressed either with N- or C-terminal EGFP-tag. The cipher in the vector name indicates the linker length of the fusion proteins (e.g. for vector pG-20-hCenpC the linker length is 20 amino acids). 

Snapshot of a simulation of the inner kinetochore model based on RMSD and paid RMSD distance metrics.

Application
The kinetochore EGFP fusion proteins can be used in modern fluorescence microscopy techniques to determine
  • localization
  • binding
  • dynamics, and, when a second color is available
  • protein-protein interactions and
  • protein-protein proximities
applying fluorescence methods like FRET, FRAP, FCS, RICS, FCCS, and F3H.

Please note
  • For FRET analysis we suggest fusion proteins with short linker
  • The fusion protein EGFP-CENP-H (expressed from vector pG-14-hCenpH) does not localize to the kinetochore. However, it can be used for single protein studies