PetakaG3^TM HOT (High Oxygen Transfer)

PetakaG3 is a new cell culture device with unique features. It has two usable surfaces, each with a growth area of 75cm² (equivalent to a T75 flask) for a total growth area of 150cm².

This compact cell management device has the same small footprint as a microtiter plate.

PetakaG3 features:

• No additional CO2 required for any type of media, although the PetakaG3™device can also be used in CO2 incubators
• No added humidity required (not influenced by environmental dehydration)
• The most secure cell culture device against contamination available today (no caps, no openings, no spills)
• Robust structure (without breakable films)
• Designed to transport live cells without requiring freezing and dry ice
• Optimizes media and growth factor supplies
• Offers 8 times more media volume per incubator capacity
• Can provide up to 15 times more cell culture surface area per incubator capacity
• Both PetakaG3 surfaces may be viewed together using any type of light microscope
• In vitro cell dormancy is easily accomplished
• Allows storage of pre-filled units that are ready to host cells
• Easy to handle. Operates in any position. Completely safe & versatile
• Each Petaka is individually bar coded for easy tracking avoiding cell damage and facilitating cell differentiation.

PetakaG3 formats:

PetakaG3™ HOT

• PetakaG3™ HOT bioreactor has the same size and structure characteristics as the PetakaG3™ LOT bioreactor.
• The PetakaG3™ HOT bioreactor is manufactured from a special co-polymer which has a particularly high diffusion rate of oxygen and carbon dioxide through the wall of the device.
• The PetakaG3™ HOT bioreactor is particularly suited for culturing cells with a high aerobic metabolism, for example, insect cells carrying the baculovirus or HEPG2, or virus-carrying cell cultures.
• Especially adapted to virus-carrying cell cultures (in vitro virus production).

PROTOCOLS

Transfection Applications: Magnetofection and Lipofection

Transfection can be achieved on both sides of a PetakaG3 cell culture device, with the same or different plasmids, allowing very sophisticated experiments on cell interactions through soluble factors and contact factors.

Magnetofection: Magnetic assisted transfection of cultured cells using PolyMag Neo (an OZ Biosciences Magnetofection-based reagent). CLICK HERE FOR PROTOCOL

Lipofection: Lipid-based transfection using DreamFect Gold^TM (an OZ Biosciences lipid-based transfection reagent). CLICK HERE FOR PROTOCOL

Filling Petaka

Cell Seeding with Tips

Hypoxic Conditions in Petaka

Culturing Adherent Cells in Petaka

Introducing Trypsin to Petaka