NICE® Expression System for Lactococcus lactis

NICE® Expression System for Lactococcus lactis

The effective & easy-to-operate
NIsin Controlled gene Expression system

Controlled gene expression in L. lactis
an emerging alternative for recombinant protein production

Features

• Expression of membrane proteins
• Tightly controlled gene expression allows production of toxic proteins
• Secretion of proteins into the medium
• Less endogenous and no exogenous proteases
• No inclusion bodies
• No spores
• Endotoxin free food grade expression system
• Simple fermentation, scale-up and down stream processing

Areas of application

• Over-expression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products  
• Expression of prokaryotic and eukaryotic membrane proteins
• Protein secretion and anchoring in the cell envelope
• Expression of genes with toxic products and analysis of essential genes
• Metabolic engineering
• Large scale applications
• Production of exo-polysaccharides
• Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
• Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as e.g. dehydrogenases and in-situ co-factor regeneration
• High throughput screening for enzyme evolution or enzyme comparison.

Description of NZ3900 expression strain: Lactococcus lactis NZ3900 – lacF-, pepN::nisRnisK; Standard strain for food grade selection based upon the ability to grow on lactose. This strain is a progeny of NZ3000, a strain in which the lactose operon, that is generally present on plasmids, has been integrated into the chromosome and the lacF gene was deleted. Deletion of the lacF gene makes this strain unable to grow on lactose unless lacF is provided on a plasmid (de Ruyter et al., 1996a).

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