NICE® Expression System for Lactococcus lactis

NICE® Expression System for Lactococcus lactis

The effective & easy-to-operate
NIsin Controlled gene Expression system

Controlled gene expression in L. lactis
an emerging alternative for recombinant protein production

Features

• Expression of membrane proteins
• Tightly controlled gene expression allows production of toxic proteins
• Secretion of proteins into the medium
• Less endogenous and no exogenous proteases
• No inclusion bodies
• No spores
• Endotoxin free food grade expression system
• Simple fermentation, scale-up and down stream processing

Areas of application

• Over-expression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products  
• Expression of prokaryotic and eukaryotic membrane proteins
• Protein secretion and anchoring in the cell envelope
• Expression of genes with toxic products and analysis of essential genes
• Metabolic engineering
• Large scale applications
• Production of exo-polysaccharides
• Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
• Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as e.g. dehydrogenases and in-situ co-factor regeneration
• High throughput screening for enzyme evolution or enzyme comparison.

Description of pNZ9530 nisRnisK vector, in strain NZ9000: pNZ9530 – Low copy plasmid with pAMβ1 origin of replication, which carries the nisR and nisK genes. For cloning in Lactococcus strains and in strains of other lactic acid bacteria genera that do not have the regulatory genes integrated into the chromosome. In this case for nisin induced expression a two plasmid system is used: e.g. pNZ9530 (nisRnisK) + pNZ8150 (+insert) (Kleerebezem et al., 1997).

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