NICE® Expression System for Lactococcus lactis

NICE® Expression System for Lactococcus lactis

The effective & easy-to-operate
NIsin Controlled gene Expression system

Controlled gene expression in L. lactis
an emerging alternative for recombinant protein production

Features

• Expression of membrane proteins
• Tightly controlled gene expression allows production of toxic proteins
• Secretion of proteins into the medium
• Less endogenous and no exogenous proteases
• No inclusion bodies
• No spores
• Endotoxin free food grade expression system
• Simple fermentation, scale-up and down stream processing

Areas of application

• Over-expression of homologous and heterologous genes for functional studies and to obtain large quantities of specific gene products  
• Expression of prokaryotic and eukaryotic membrane proteins
• Protein secretion and anchoring in the cell envelope
• Expression of genes with toxic products and analysis of essential genes
• Metabolic engineering
• Large scale applications
• Production of exo-polysaccharides
• Production of ingredients through metabolic engineering: e.g. alanine, folate, diacetyl
• Preparation of L. lactis as a biocatalyst by expression of a suitable enzyme as e.g. dehydrogenases and in-situ co-factor regeneration
• High throughput screening for enzyme evolution or enzyme comparison.

Description of pNZ8008 expression vector: pNZ8008 – Reference plasmid for testing the nisin induction in Lactococcus and other lactic acid bacteria genera. A gusA gene without promoter was fused to the nisin A promoter (PnisA) (de Ruyter et al., 1996).

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