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Mobicols & Lab Columns

SCO000 - MobiSpin Columns prefilled with Gel Filtration Matrix, 20/pack



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Gel Filtration Matrix

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MobiSpin S-Columns pre-packed with S-200, S-300 and S-400 Sephacryl® HR matrices are designed for a wide variety of separation tasks such as nucleic acid purification. These columns are easy to handle with reproducible results and a fast, simple procedure that can be completed in under 4 minutes. They function by the proven principle of size-exclusion chromatography. The columns combine the effectiveness of gel filtration with the speed of centrifugation. The pore size of the filled-in matrix determines which molecules are small enough to enter the pores of the matrix beads and which molecules are too large. 

MobiSpin G-Columns pre-packed with Sephadex® G-50 resin remove small molecules from DNA/RNA, such as salt ions, and are therefore most suitable for buffer exchange. The Sephadex G-50 matrix is a hydrophilic matrix that swells in buffer and acquires its chromatographic properties under wet conditions. In this state, beads have a pore size of 700 Da and allow hydrated salt ions or dNTPs (dye-labeled or radiolabeled) to enter the pores while DNA/RNA under 20 bases and most other biomolecules stay outside. 

The MobiSpin S-Columns and G-Columns are closed with a screw cap and the tip is a fixed snap-off plug.

The resin is provided pre-packed in MobiSpin columns


  • MobiSpin S-Columns - Pre-packed with Sephacryl® HR resin and equilibrated in MobiSpin Buffer (10 mM Tris/HCl pH 7.6; 1 mM EDTA)
  • MobiSpin G-Columns - Pre-packed with Sephadex® G-50 resin and equilibrated in STE buffer (10mM Tris/HCl pH 8.0, 100 mM NaCl, 1 mM EDTA)
  • Sample purification can be completed in less than 4 minutes
  • Creates reproducible results with simplified protocols
  • Numerous samples can be processed simultaneously
  • No sample dilution
  • Compatible with laboratory standards


  • SCO200 - MobiSpin column prefilled with S-200 x 20
  • SCO300 - MobiSpin column prefilled with S-300 x 20
  • SCO400 - MobiSpin column prefilled with S-400 x 20
  • SCO500 - MobiSpin column prefilled with G-50 x 20