Agarose Gel DNA Extraction

■ Description

DNA fragments for probe DNA or ligation must be separated and purified from other DNA fragments. MEGA-bead employs a bead method to purify target DNA in excised agarose gel. The bead method uses a highly concentrated salt solution to keep the target DNA bound to the bead and prevent the target DNA from solubilization. The binding reaction occurs due to the disruption of the organized structure of water molecules and the interaction with the nucleic acids. Thus the adsorption to the specifically pretreated spherical silica matrix is favored. Since the binding process is specific for nucleic acids, the bound material can be separated and purified from impurities e.g. salts and proteins, by a simple washing step. Nucleic acids elute from the matrix in a low salt buffer or water. The size of MEGA-bead is much smaller than other companies' products to yield more surface area for DNA binding, and guarantees high yield of purification up to 80-90%. DNA fragments isolated with MEGA-bead Agarose Gel Extraction Kit are efficiently ligated into plasmid cloning vectors or specifically labeled using either random primed labeling or nick translation. No inhibition of digestion with restriction endonucleases is observed. Pure DNA can be obtained ithin 15-20 minutes.

■ Applications

Sequencing, cloning, ligation, probe labeling ligation, random primed labeling, nick translation, etc.

■ Characteristics

• DNA fragments extracted in 20 minutes
• Gel extraction from standard or low-melt gels in TAE or TBE buffer
• Does not use sodium iodide
• No change of pH, sodium acetate addition not required
• High recovery and purity
• No interfere with subsequent reactions

■ Packaging: 100 purifications per kit.