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Automated Magnetic Bead Based Purification of Nucleic Acids

MD61021 - MagSi-NGSPREP; 75ml

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A Simple and Quick Way to Consistent Next Generation Sequencing Results


MagSi-NGSPREP is designed for an optimized purification of next generation library preparation through efficient clean up of the successive enzymatic reactions (i.e. end repair, dA-tailing, adaptor ligation). MagSi-NGSPREP is used for clean-up of library targeting sequencers such as Life Technologies' ABI SOLiD, Ion Torrent; Illumina's HiSeq 2000, MiSeq, GAIIx; and Roche's 454.

Magnetic bead based MagSi-NGSPREP offers an efficient solution for clean-up and size selection in library preparation of NGS sequencing reactions. The simple and flexible protocol can be adjusted to your specific application and NGS platform. MagSi-NGSPREP can be used manually but is also easy to automate for high-throughput processing. In conclusion: a simple and quick way to consistent NGS results. 

Features:

High quality results
  • High recovery of DNA fragments, more than 70%
  • Excellent removal of enzymes, primers, oligonucleotides, polymerase, and other contaminants
  • Fragment size selection adjustable between 100 and 1000 base pairs
  • Guarantees consistent sequencing results
Simplify your routine
  • One product for all clean-up and size selection steps in the library preparation workflow
  • Simple bind - wash - elute procedure
  • Protocol easily adjustable for clean-up or size selection using specific reagent-to-sample ratios
  • Manual and automated use
  • Meets the requirements of common library preparation kits
Easy to automate
  • Fast and robust protocol resulting in a high-throughput on automated systems
  • Optimized separation performance using validated magnetic separators for 96 and 384 wells PCR plates
  • Compatible with many different automated liquid handling systems (e.g. PerkinElmer®, Agilent Technologies®, Beckman Coulter®)
Gel electrophoresis of DNA ladder at different reagent:sample volume ratios.
The size of fragments eluted from the beads (or that bind in the first place) is determined by the end concentration of MagSi-NGSPREP, and this in turn is determined by the mix of DNA and beads.
A 50µl DNA sample plus 75µl of beads will give MagSi-NGSPREP:DNA ratio of 1.5. As this ratio is changed, the length of fragments binding and/or left in solution also changes. Hence, the lower the ratio of MagSi-NGSPREP:DNA the larger the final fragments will be at elution. 
Smaller fragments are retained in the buffer so you get different size ranges from a single sample with multiple purifications. Part of the reason for this effect is that DNA fragment size affects the total charge per molecule with larger DNAs having larger charges; this promotes their electrostatic interaction with the beads and displaces smaller DNA fragments. 



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