Automated Magnetic Bead Based Purification of Nucleic Acids

The MagSi-gDNA blood kit allow fast and economic automated purification of genomic DNA from a wide range of sample volumes (20µl up to 1ml) of fresh or frozen whole blood samples using magnetic beads and any liquid handling workstation. The scalability of the MagSi-gDNA blood kits makes it possible to use the products in any situation where gDNA isolation out of blood is needed from single experiments using the MM-Separators leading though to high throughput platforms of which the KingFisher® is a perfect and widely used example. 

In combination with the liquid handling systems the automated MagSi-gDNA blood kits enable purification of DNA from 1-96 samples of whole human blood or other commonly used blood components (buffy coat, serum). After filling the standard containers (single tubes, 96 deepwell plates), all required for isolation steps are robottically performed. In contrast to other blood kits available using blood volumes > 1ml, lysis of erythrocytes is not required. Blood sample lysed with the MagSi-gDNA blood kit is optimal as the lysis buffer contains Proteinase K. 

In combination with the robotic workstation the lysis step can be carried out direct on the platform at ambient temperature or via the integrated thermomixer available for most robots at 56°C. Further processing is done by making use of the magnetic capabilities of the MagSi DNA beads.

The free DNA binds to magnetic particles, followed by washing steps and the final elution. The high quality DNA can be stored at -20°C or is ready to use for subsequent downstream applications.