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LPS Extraction Kit

17141 - LPS Extraction Kit

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Our LPS Extraction Kit is designed to separate and purify Lipopolysaccharides (LPS) present in the outer wall of gram-negative bacteria. This kit can extract LPS from even a small volume of bacterial cells and gives reproducibly high yields of LPS. The protocol takes less than 60 minutes and includes only three steps: lysis of bacterial cells, purification of LPS among the released cell components, and washing to remove salts. This kit provides 100 extractions.

Note: The yield of LPS extraction is proportional to the volume of the culture. The yield of LPS is at its maximum when 5ml of culture is used. The optimal culture volume is 5ml at OD600 of 0.8-1.2.

protocol

Features

  • Isolates and purifies LPS present in gram negative bacteria 
  • Broadly applicable among different gram-negative bacteria -Removes contaminants such as proteins and nucleic acids 
  • Extraction is completed in under 60 minutes

Applications

  • Study of LPS composition and structure
  • Systematic study of bacteria
  • Study of immune response of carbohydrate antigen
  • LPS inhibition drug design study
  • Antibiotic target study

Contents

  • Lysis buffer, 100ml
  • Purification buffer, 80ml

Literature