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B. megaterium T7 RNA Polymerase High Yield Gene Expression

BMEGT702 - B. megaterium protoplasts strain MS941, pretransformed with pT7-RNAP, 5 X 500ul

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The B. megaterium protoplasts strain MS941, pretransformed with pT7-RNAP, can be ordered separately or as part of the complete gene expression kit.

Click Here for the T7 Handbook and User Manual

This video shows a growing micro-colony of cells of Bacillus megaterium MS941 expressing GFP observed in a Zeiss Axiovert 200 M microscope. The cells are induced shortly before the film starts. A brightfield image and a fluorescence image are recorded every 5 minutes over a timespan of 5 1/2 hours. For this film the brightfield images are overlayed by the green coloured fluorescence images. Recorded by Stefan Leupold, Institut für Mikrobiologie, Technische Universität Braunschweig.