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B. megaterium T7 RNA Polymerase High Yield Gene Expression

BMEGT710 - B. megaterium pPT7 cloning vector, lyophilized, 10ug

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The B. megaterium pPT7 cloning vector is a component of the B. megaterium high yield T7 gene expression kit.

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This video shows a growing micro-colony of cells of Bacillus megaterium MS941 expressing GFP observed in a Zeiss Axiovert 200 M microscope. The cells are induced shortly before the film starts. A brightfield image and a fluorescence image are recorded every 5 minutes over a timespan of 5 1/2 hours. For this film the brightfield images are overlayed by the green coloured fluorescence images. Recorded by Stefan Leupold, Institut für Mikrobiologie, Technische Universität Braunschweig.