Tag-Binding Beads

Nickel His-tag Affinity Purification IMAC Beads

Recombinant proteins tagged with 6-10 poly Histidines can be purified in one step by ion metal affinity chromatography (IMAC). Several metal ions exhibiting affinity to poly-Histidines are commonly used, Nickel and Cobalt being the most popular. Tagging the proteins may be done at the N-ter or the C-ter end by cloning a gene in dedicated expression vectors encoding the poly Histidines stretch, enabling the purification of the resulting recombinant protei. Adar's Nickel Beads are a high quality Nickel affinity-resin product, that exhibits high capacity and good selectivity towards His-tagged proteins. Purification with Adar's Nickel Beads may be done in a variety of formats, such as gravity-flow columns and small or large-scale batches. The preferred purification strategy is to start by using the native purification conditions and only later on, if required, use denaturing conditions for proteins that are accumulated as inclusion bodies or in cases when the 6xHis purification tag is not exposed in the native form. The amount of actual protein bound can vary with the type and size of protein. Reducing agents such as DTT and chelating agents such as EDTA and EGTA, may be used in the buffers employed in the extraction protocols, but not during the affinity purification itself. Gel filtration or dialysis are recommended procedures for removal of these agents.

Nickel Beads Specifications