Tag-Binding Beads

Glutathione GST-tag Affinity Purification Beads

Adar Biotech's Glutathione Beads are used for the purification of glutathione Stransferase (GST) tagged proteins, produced using various commercial expression vectors. GST-fused proteins can be purified from bacterial lysates by one-step affinity purification. Adar biotech Glutathione Beads is state of the art affinity-resin product that consists of glutathione attached through sulfur to an epoxy-activated 4% cross-linked beaded agarose. The use of the Glutathione Beads enables the purification of various GSTfused proteins, while maintaining mild, non-denaturing conditions throughout the purification process.

Matrix: Sepharose CL-4B

Activation method: Oxiran

Binding capacity: 4-8 mg/ml recombinant GST

Bead size: 40-165 μm

Bead structure: Highly cross-linked spherical agarose, 4%

Max back pressure: 0.3 MPa, 3 bar

Max. flow rates: 4 ml/min/cm²

Recommended flow rate: 1-2 ml/min/cm²

Stability of the matrix: pH 4-10

Storage: 4°C in PBS pH 7.4 added with NaN3 0.1% (w/v) as preservatives