Genomic DNA Isolation

It is difficult to extract DNA from plants because plants contain polysaccarides and polyphenolic compounds which inhibit downstream applications, including PCR. The G-spin IIp Kit utilizes buffers that specifically remove polysaccharides and other secondary metabolites and yields highly purified DNA that is free of inhibitors and suitable for PCR, Southern blotting, RAPD, restriction analysis, GMO detection and sequencing applications.

The entire procedure, including cell lysis, takes less than 1 hour and consists of the following steps: ● grind frozen sample in a homogenizer ● add supplied buffers and incubate for 30 minutes (lysis step) ● precipitate to remove contaminants ● purify using G-spin IIp columns and elute

The G-spin IIp Kit is applicable to a wide variety of plant species and food samples. For HIGH PURITY + HIGH YIELDS and removal of inhibitory metabolites, try G-spin IIp.