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FlyFectin Insect Cell Transfection

FF50000 - FlyFectin Insect Cell Transfection Reagent

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Starting at: $189.00

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FlyFectin™ Insect Cell Transfection Reagent is a powerful reagent designed for the highly efficient, reproducible transfection of insect cells using TEE (Triggered Endosomal Escape) technology. TEE technology combines and exploits the properties of lipids and polymers to achieve efficient DNA delivery into cells.  FlyFectin™ can be used with all types of nucleic acids. It has many applications, including the production of recombinant proteins using the Baculovirus expression system. This reagent has been effectively used with many insect cells including Ag55, Anso, Asd43, Bm5, Cl8, Cpp512, High5, IPBL-SF21, Kc167, Ld652, Mos20, S2, Sf9, SL-2, SL-3, and SPC-SL52.

Figure 1: Transfection of various insect cell lines with FlyFectin. Cells were transfected in 6 well plate (LD652 and SL2) or in 25cm2 flask  (S2 and Bm-5) with 2 μg of DNA (6 well) or 15-20μg DNA (flask) and 5 to 6 μl of FlyFectin per μg of DNA. Transfection efficiency was monitored 18 to 72 h after transfection.


Figure 2: Comparison between FlyFectin and other transfection reagents. Cells were transfected according to the instruction manuals. Luciferase activities were measured with a Luciferase assay kit and results are expressed as relative values.

Features

  • Specialized for insect cells
  • High transfection efficiency and excellent reproducibility
  • Fast and easy procedure
  • Compatible with and without serum-containing culture media
  • Can be used with all types of nucleic acids
  • Non-toxic

Applications

  • Transfection of insect cells

Sizes

  • FF50500– FlyFectin Transfection Reagent, 500 µl (125 transfections)
  • FF51000 - FlyFectin Transfection Reagent, 1ml (250 transfections)
  • FF55000 – FlyFectin Transfection Reagent, 5ml (500 transfections)