FlyFectin is a specifically designed and very efficient transfection reagent for insect cells. Innovative and unique, FlyFectin is:
- Highly efficient with excellent reproducibility
- Multipurpose (various types of nucleic acids)
- Ideal for recombinant protein production with Baculovirus expression system
- Simple, Ready-to-use and Rapid
- Compatible with and without serum-containing culture media
- Non toxic
- Economical
FlyFectin Offers A Unique Solution
Based on an aqueous formulation of cationic and neutral lipids, FlyFectin has been developed exclusively to transfect insect cells. FlyFectin is perfect to produce high level of recombinant protein in
association with a Baculovirus expression system. High transfection efficiencies are also accomplished in a wide variety of insect cells with various types of nucleic acids.
Highly Efficient FlyFectin Reagent
The complexes formed by DNA and FlyFectin reagent have an exceptional capacity to destabilize cells membrane, allowing the delivery of important DNA amounts into the cytosol. Transfection is very efficient, and
highly reproducible, even with very low amounts of nucleic acids. Consequently, FlyFectin is the ideal reagent for numerous insect cells.
Universal and Multipurpose
FlyFectin is adapted to the delivery of DNA, mRNA, siRNA or oligonucleotides and has been successfully used on various insect cells.
FlyFectin can be used for all applications: stable and transitory transfection, Co-transfection, high throughput screening... FlyFectin is the ideal reagent for production of recombinant protein using
Baculovirus expression system.
Flyfectin is compatible with and without serum-containing culture media.
No Toxicity
Flyfectin is a non-toxic gene delivery method. Due to its biochemical and biophysical properties, FlyFectin formulation is highly efficient at low doses and the potential secondary effects are avoided.
Simplicity & Quickness
Flyfectin is a simple and ready to use reagent. Transfection is achieved in a 2-step process:
1: Mix DNA and FlyFectin
2: Add to your cells
500μl of FlyFectin is sufficient for 125 transfections (1μg DNA).
Download our FlyFectin User Manual
Cell Lines Tested
Cell Lines | Cell Type |
Cl8 | Drosophila |
High5 | Trichoplusia |
KC167 | Drosophila |
S2 | Drosophila |
Sf9 | Spodoptera frugiperda ovarian cells |
SL-3 | Spodoptera frugiperda ovarian cells |
SPC-SL 52 | Spodoptera littoralis ovarian cells |
