"EnBase Technology...enzyme based substrate delivery...uses an enzyme to release glucose from a solubilized polysaccharide in a slow and consistent manner to reach greater protein product yields"
EnBase® Flo Yeast Medium
EnBase Flo Yeast Medium is designed to simplify the screening of transformations, growth of seeding cultures and biomass production.
Description
EnBase® Flo Yeast Medium is a pre-sterilized medium with complex additives to obtain high cell mass in shake flask or multiwell plate cultivations of various yeast species and strains. The principle is based on the patented EnBase Technology. This technology uses an enzyme to degrade a solubilized polysaccharide within the medium to release glucose in a slow and consistent manner. The enzymatic control of glucose delivery is governed by the amount of enzyme used and provides an optimal cultivation environment for growth. This controlled growth allow cultures to reach greater cell densities compared to traditional media because it supports favourable pH and stable oxygen transfer rate. EnBase Flow Yeast Medium simplifies screening of transformations, growth of seeding cultures, and biomass production of yeast.
Ultra Yield Flasks and AirOtop Enhanced Seals are recommended for appropriate oxygen transfer:
Ultra Yield Flasks are optimized with specialized seals to enhance the oxygenation in shake flask cultures. These sterile and disposable flasks serve as a replacement to the standard, less effective shake flask. Click here for size options.
Click Here for Protocol
EXAMPLE: EnBase® technology:
a powerful tool for yeast biotransformations
Screening of microbial whole cell transformations can be simplified by using the EnBase technology to obtain a high cell density culture in smaller cultivation volumes.
The EnBase® Flo Yeast Medium allowed for more efficient screening of microbial whole cell biotransformations. In a study performed in conjunction with BioWorx (Berlin, Germany), EnBase Flo Yeast medium was able to provide better performance than standard medium.
- Higher cell densities
- Increased conversion of 2-Butanone to 2-Butanol
- Enantioselectivity was improved in 15 of 20 yeast strains
- Six strains were able to reach enantioselectivity of >97% (0 in standard YED medium)
YG - Yeast extract glucose medium
BS - EnBase Flo Yeast medium (BioSilta)
Examples of the improvement seen when culturing yeast with EnBase Flo Yeast Medium compared to yeast extract glucose medium.
MoBiTec - Molecular Biotechnology
