Plasmid Purification

The DNA-midi Kit features our unique midi-bead solution (patent pending). 500µl of midi-bead solution can bind up to 150µg DNA from 10-100ml of E. coli culture. After E. coli lysis, DNA is bound to the midi-beads and then washed and eluted using our special spin columns. You get simple, straightforward isolation of high purity plasmid DNA in less than 1 hour!

FEATURES:

•  midi scale: 10ml-100ml E. coli culture
• up to 150µg DNA per prep
• OD 260/280 = 1.7-2.0
• works with both endA+ and endA- E. coli strains
• Total Prep Time < 1 Hour

DOWNSTREAM APPLICATIONS:

• sequencing
• cloning
• enzyme digestion
• ransfection
• in vitro transcription/translation

PACKAGING: 50 preps per kit

NOTE: A Maxi protocol is included for extracting up to 600ug of DNA