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DNA Polymerases/SNPase

101000 - DFS Taq DNA Polymerase DNA FREE!

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Bioron DFS Taq DNA Polymerase - the "cleaner" DNA-Free, Sensitive Taq Polymerase

Description: Taq DNA Polymerase is a thermostable enzyme of approximately 94kDa isolated from the eubacterium Thermus aquaticus strain YT-1. This unmodified enzyme replicates DNA at 72şC. The enzyme catalyzes the polymerization of nucleotides into duplex DNA in the 5' to 3' direction in the presence of magnesium ions and possesses a 5' to 3' exonuclease activity. The enzyme is highly purified and is free of nonspecific endo- or exonucleases. Taq DNA polymerase leaves single 3'-dA overhangs on the reaction products.

Features:

  • This Taq DNA Polymerase is absolutely free from bacterial DNA
  • With BIORON buffer system for all purposes suitable
  • High sensitivity: 6 molecules template are enough
  • Efficient amplification of single copy genes
  • High yields of products

Performance and purity tests: DFS Taq DNA polymerase effectively directs primer extension with templates up to 5Kb in length. The enzyme was tested for the absence of nuclease and nickase activity. No traces of bacterial DNA were detected in a reaction without template, tested with primers complementary to the conserved region of the 16S ribosomal gene. Note: In contrast to DFS enzyme, Taq DNA polymerase from a variety of other suppliers contain contaminating DNA: with DNA-contaminated Taq DNA polymerase one should be ready to observe false positive PCR results in some cases.

DFS

E. coli DNA Test with DFS- and another Taq Polymerase using E.coli 16S RNA specific primers:
1 - without adding E.coli DNA (Test)
2 - with adding E.coli DNA (control)

Sensitivity: The sensitivity of DFS Taq Polymerase is very high, with a detection level of 10-100 copies of DNA.

Concentration: 5 units/ul

10X Reaction Buffers:Two different buffers are provided: Complete with MgCl2 included in the buffer; and Incomplete without MgCl2 in the buffer, but with a separate vial of MgCl2 so that you can control the concentration.


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