Magnetofection Fluorescent reagents
Magnetofection™ is a simple and highly efficient method to transfect cells in culture and in vivo. The principle is to associate nucleic acids, transfection reagents or viruses with specific cationic magnetic nanoparticles. The resulting complexes are then concentrated and transported into cells supported by an appropriate magnetic field. In this way, the exploitation of a magnetic force exerted upon gene vectors allows a very rapid concentration of the entire applied vector dose on cells, so that 100% of the cells get in contact with a significant vector dose. OZ Biosciences offers four types of ready-to-use reagents (CombiMag, PolyMag, SilenceMag, and ViroMag) and their fluorescent equivalents.
Red FluoMag reagents are tetramethylrhodamine-conjugated magnetic nanoparticles. These red fluorescent reagents are useful for many applications:
Double labeling and co-localization studies using GFP or FITC labeled nucleic acidsFACS analysis, fluorescent and confocal microscopyTransfection mechanisms (follow interaction with cells, intracellular pathway, ...)Fluorescent resonance energy transfer (FRET) assay as well as tracking internalization pathway in endocytic vesiclesDetermine complexes stability in various biological environmentAnalyze the association of nucleic acids or transfection reagents or viruses with the magnetic nanoparticlesReagents available
FluoMag-C corresponding to CombiMagFluoMag-S corresponding to SilenceMagFluoMag-V corresponding to ViroMagFluoMag-P corresponding to PolyMag Transfection (plasmid DNA) with FluoMag-P
